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ex taq dna polymerase  (TaKaRa)


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    Structured Review

    TaKaRa ex taq dna polymerase
    Ex Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 16023 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ex+taq+polymerase/TaKaRa+Ex+Taq+DNA+Polymerase/pmc13090712-41-0-7
    Average 98 stars, based on 16023 article reviews
    ex taq dna polymerase - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Apple consumption is associated with obesity- and lipid-related parameters and gut microbiota profiles across enterotypes: 12-week single-blind trial in Japanese adults
    Article Snippet: .. The PCR amplification was performed in a 10 μL reaction mixture containing 1 μL of 10 × Ex buffer (TaKaRa Bio, Otsu, Shiga, Japan), 0.8 μL dNTPs (TaKaRa Bio), 0.5 μL each of the forward and reverse primers, 2 μL template DNA, and 0.1 μL of Ex Taq polymerase (TaKaRa Bio). .. Thermal cycling conditions were as followed: initial denaturation at 94 °C for 2 min; 30 cycles of 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s; followed by a final extension at 72 °C for 5 min. Amplified products were purified using an AMPure XP Beads Kit (Beckman Coulter, Inc., Brea, CA, United States) according to the manufacturer’s instructions, and the product quality was verified by agarose gel electrophoresis.

    Article Title: Host regulation of gill microbiota by a mucosal C-type lectin in the kuruma shrimp Penaeus japonicus
    Article Snippet: Mucosal organs are the primary interface through which shrimp are exposed to environmental microorganisms and pathogens, however, our understanding of crustacean mucosal immunity and its relationship with resident microbiota remains limited.. In this study, we examined the role of a mucosa-specific C-type lectin, MjGCTL, in the regulation of mucosal microbiota and disease susceptibility in kuruma shrimp Penaeus japonicus.. MjGCTL mRNA expression was detected in the gill, stomach, and hindgut, which are recognized as mucosal organs in shrimp.

    Article Title: Microorganism of
    Article Snippet: .. For genetic manipulation, Ex Taq polymerase (Takara) and Pfu polymerase (Solgent) were used as PCR polymerases, and various restriction enzymes and DNA modifying enzymes purchased from NEB were used, and they were used according to manufacturer's provided buffers and protocols. ..

    Article Title: Compartment-resolved in vivo phage display biopanning reveals constraint-driven peptide sequence landscapes
    Article Snippet: .. The primer sequences used were: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGttggtgccggcaac-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGgccctctatagttagc-3′ PCR reactions were performed using the following reaction mixture: 5 μl PCR buffer, 4 μl dNTP mix, 1 μl each of forward and reverse primers (10 μM), 0.3 μl Ex Taq polymerase (Takara Bio, Kusatsu, Japan) and template DNA (1 μl for SM buffer samples or 5 μl for LCM samples), with nuclease-free water added to a final volume of 50 μl. .. Thermal cycling conditions were as follows: initial denaturation at 94°C for 3 min; 30–45 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 30 s; followed by a final extension at 72°C for 7 min. PCR products were separated by electrophoresis on 1.5% agarose gels, and DNA fragments of the expected size were excised under blue LED illumination and purified using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio).

    Article Title: Genome-Scale Phylogenetic Evidence Supports the Synonymy of Lasiodiplodia brasiliensis with Lasiodiplodia theobromae
    Article Snippet: .. PCR reactions were performed in 10 μL volumes, consisting of 1 μL of 10× Taq buffer, 0.8 μL of dNTPs (2.5 mM each), 0.1 μL of each primer, 0.05 μL of Ex Taq polymerase (TaKaRa Ex TaqTM, Takara, Shiga, Japan), 7 μL of double-distilled water, and 1 μL of genomic DNA. ..

    Article Title: Genome-Scale Phylogenetic Evidence Supports the Synonymy of Lasiodiplodia brasiliensis with Lasiodiplodia theobromae
    Article Snippet: .. PCR reactions were performed in 10 μL volumes, consisting of 1 μL of 10× Taq buffer, 0.8 μL of dNTPs (2.5 mM each), 0.1 μL of each primer, 0.05 μL of Ex Taq polymerase (TaKaRa Ex TaqTM, Takara, Shiga, Japan), 7 μL of double-distilled water, and 1 μL of genomic DNA. ..

    Article Title: Evaluation of Predation on Phytophagous Insects by a Phytozoophagous Mirid Bug, Apolygus lucorum .
    Article Snippet: Primers for A. gossypii: Forward primer MYF: 5′-TTCACATCAGCAACTATAATC-3′ Reverse primer MYR: 5′-ACTACATAATAAGTGTCATGC-3′ Amplicon length: 208 bp. (c) PCR amplification: PCR reactions were performed in a thermal cycler (Bioer, Hangzhou, China). .. Each 25 μL reaction mixture contained 0.25 μL of EX Taq polymerase (TaKaRa, Dalian, China), 2.5 μL of 10× PCR buffer (supplied with the Taq polymerase), 2 μL of dNTPs (Tiangen Biotech, Beijing, China), 1 μL of DNA template, 0.5 μL of each forward and reverse primer, and sterile distilled water to a final volume of 25 μL. ..

    Amplification:

    Article Title: Apple consumption is associated with obesity- and lipid-related parameters and gut microbiota profiles across enterotypes: 12-week single-blind trial in Japanese adults
    Article Snippet: .. The PCR amplification was performed in a 10 μL reaction mixture containing 1 μL of 10 × Ex buffer (TaKaRa Bio, Otsu, Shiga, Japan), 0.8 μL dNTPs (TaKaRa Bio), 0.5 μL each of the forward and reverse primers, 2 μL template DNA, and 0.1 μL of Ex Taq polymerase (TaKaRa Bio). .. Thermal cycling conditions were as followed: initial denaturation at 94 °C for 2 min; 30 cycles of 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s; followed by a final extension at 72 °C for 5 min. Amplified products were purified using an AMPure XP Beads Kit (Beckman Coulter, Inc., Brea, CA, United States) according to the manufacturer’s instructions, and the product quality was verified by agarose gel electrophoresis.

    Laser Capture Microdissection:

    Article Title: Compartment-resolved in vivo phage display biopanning reveals constraint-driven peptide sequence landscapes
    Article Snippet: .. The primer sequences used were: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGttggtgccggcaac-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGgccctctatagttagc-3′ PCR reactions were performed using the following reaction mixture: 5 μl PCR buffer, 4 μl dNTP mix, 1 μl each of forward and reverse primers (10 μM), 0.3 μl Ex Taq polymerase (Takara Bio, Kusatsu, Japan) and template DNA (1 μl for SM buffer samples or 5 μl for LCM samples), with nuclease-free water added to a final volume of 50 μl. .. Thermal cycling conditions were as follows: initial denaturation at 94°C for 3 min; 30–45 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 30 s; followed by a final extension at 72°C for 7 min. PCR products were separated by electrophoresis on 1.5% agarose gels, and DNA fragments of the expected size were excised under blue LED illumination and purified using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio).

    Sterility:

    Article Title: Evaluation of Predation on Phytophagous Insects by a Phytozoophagous Mirid Bug, Apolygus lucorum .
    Article Snippet: Primers for A. gossypii: Forward primer MYF: 5′-TTCACATCAGCAACTATAATC-3′ Reverse primer MYR: 5′-ACTACATAATAAGTGTCATGC-3′ Amplicon length: 208 bp. (c) PCR amplification: PCR reactions were performed in a thermal cycler (Bioer, Hangzhou, China). .. Each 25 μL reaction mixture contained 0.25 μL of EX Taq polymerase (TaKaRa, Dalian, China), 2.5 μL of 10× PCR buffer (supplied with the Taq polymerase), 2 μL of dNTPs (Tiangen Biotech, Beijing, China), 1 μL of DNA template, 0.5 μL of each forward and reverse primer, and sterile distilled water to a final volume of 25 μL. ..



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    Comparison of the genome organization between Apodemus agrarius coronavirus (AaCoV) 1 Aa18–157 and representative betacoronaviruses (β-CoV). The predicted ORFs and conserved domains are represented by boxes in the following colors: orange for non-structural proteins, blue for structural proteins, and green for accessory proteins. AaCoV-1 Aa18–157 and rodent-borne β-CoVs display a genomic structure and gene organization typical of CoVs, arranged from 5′ to 3′ as follows: non-structural proteins located in the ORF1ab region; structural proteins including HE, S, E, M, and N; and accessory proteins such as NS2a, NS4, NS5, and N2. Abbreviations: ORF, open reading frame; NS, non-structural protein; 3CL, 3CL main protease; RdRp, RNA-dependent RNA <t>polymerase;</t> Hel, helicase; HE, haemagglutinin esterase; S, spike; E, envelope; M, membrane; N, nucleocapsid; I, internal; CoV, coronavirus; AaCoV, Apodemus agrarius CoV; ChRCoV, China Rat CoV; ApCoV, Apodemus peninsulae CoV; RaCoV, Rattus argentiventer CoV; RtCoV, Rattus tanezumi CoV; MrCoV, Myodes rufocanus CoV; HCoV, Human CoV; MHV, murine hepatitis virus; MERS, middle east respiratory syndrome; SARS, severe acute respiratory syndrome. The sequence of 5′ termini untranslated region was uncovered in AaCoV Jingmen, AaCoV Longquan-343, and RtCoV HMU-1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Comparison of the genome organization between Apodemus agrarius coronavirus (AaCoV) 1 Aa18–157 and representative betacoronaviruses (β-CoV). The predicted ORFs and conserved domains are represented by boxes in the following colors: orange for non-structural proteins, blue for structural proteins, and green for accessory proteins. AaCoV-1 Aa18–157 and rodent-borne β-CoVs display a genomic structure and gene organization typical of CoVs, arranged from 5′ to 3′ as follows: non-structural proteins located in the ORF1ab region; structural proteins including HE, S, E, M, and N; and accessory proteins such as NS2a, NS4, NS5, and N2. Abbreviations: ORF, open reading frame; NS, non-structural protein; 3CL, 3CL main protease; RdRp, RNA-dependent RNA <t>polymerase;</t> Hel, helicase; HE, haemagglutinin esterase; S, spike; E, envelope; M, membrane; N, nucleocapsid; I, internal; CoV, coronavirus; AaCoV, Apodemus agrarius CoV; ChRCoV, China Rat CoV; ApCoV, Apodemus peninsulae CoV; RaCoV, Rattus argentiventer CoV; RtCoV, Rattus tanezumi CoV; MrCoV, Myodes rufocanus CoV; HCoV, Human CoV; MHV, murine hepatitis virus; MERS, middle east respiratory syndrome; SARS, severe acute respiratory syndrome. The sequence of 5′ termini untranslated region was uncovered in AaCoV Jingmen, AaCoV Longquan-343, and RtCoV HMU-1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Comparison of the genome organization between Apodemus agrarius coronavirus (AaCoV) 1 Aa18–157 and representative betacoronaviruses (β-CoV). The predicted ORFs and conserved domains are represented by boxes in the following colors: orange for non-structural proteins, blue for structural proteins, and green for accessory proteins. AaCoV-1 Aa18–157 and rodent-borne β-CoVs display a genomic structure and gene organization typical of CoVs, arranged from 5′ to 3′ as follows: non-structural proteins located in the ORF1ab region; structural proteins including HE, S, E, M, and N; and accessory proteins such as NS2a, NS4, NS5, and N2. Abbreviations: ORF, open reading frame; NS, non-structural protein; 3CL, 3CL main protease; RdRp, RNA-dependent RNA <t>polymerase;</t> Hel, helicase; HE, haemagglutinin esterase; S, spike; E, envelope; M, membrane; N, nucleocapsid; I, internal; CoV, coronavirus; AaCoV, Apodemus agrarius CoV; ChRCoV, China Rat CoV; ApCoV, Apodemus peninsulae CoV; RaCoV, Rattus argentiventer CoV; RtCoV, Rattus tanezumi CoV; MrCoV, Myodes rufocanus CoV; HCoV, Human CoV; MHV, murine hepatitis virus; MERS, middle east respiratory syndrome; SARS, severe acute respiratory syndrome. The sequence of 5′ termini untranslated region was uncovered in AaCoV Jingmen, AaCoV Longquan-343, and RtCoV HMU-1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Comparison of the genome organization between Apodemus agrarius coronavirus (AaCoV) 1 Aa18–157 and representative betacoronaviruses (β-CoV). The predicted ORFs and conserved domains are represented by boxes in the following colors: orange for non-structural proteins, blue for structural proteins, and green for accessory proteins. AaCoV-1 Aa18–157 and rodent-borne β-CoVs display a genomic structure and gene organization typical of CoVs, arranged from 5′ to 3′ as follows: non-structural proteins located in the ORF1ab region; structural proteins including HE, S, E, M, and N; and accessory proteins such as NS2a, NS4, NS5, and N2. Abbreviations: ORF, open reading frame; NS, non-structural protein; 3CL, 3CL main protease; RdRp, RNA-dependent RNA <t>polymerase;</t> Hel, helicase; HE, haemagglutinin esterase; S, spike; E, envelope; M, membrane; N, nucleocapsid; I, internal; CoV, coronavirus; AaCoV, Apodemus agrarius CoV; ChRCoV, China Rat CoV; ApCoV, Apodemus peninsulae CoV; RaCoV, Rattus argentiventer CoV; RtCoV, Rattus tanezumi CoV; MrCoV, Myodes rufocanus CoV; HCoV, Human CoV; MHV, murine hepatitis virus; MERS, middle east respiratory syndrome; SARS, severe acute respiratory syndrome. The sequence of 5′ termini untranslated region was uncovered in AaCoV Jingmen, AaCoV Longquan-343, and RtCoV HMU-1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Comparison of the genome organization between Apodemus agrarius coronavirus (AaCoV) 1 Aa18–157 and representative betacoronaviruses (β-CoV). The predicted ORFs and conserved domains are represented by boxes in the following colors: orange for non-structural proteins, blue for structural proteins, and green for accessory proteins. AaCoV-1 Aa18–157 and rodent-borne β-CoVs display a genomic structure and gene organization typical of CoVs, arranged from 5′ to 3′ as follows: non-structural proteins located in the ORF1ab region; structural proteins including HE, S, E, M, and N; and accessory proteins such as NS2a, NS4, NS5, and N2. Abbreviations: ORF, open reading frame; NS, non-structural protein; 3CL, 3CL main protease; RdRp, RNA-dependent RNA <t>polymerase;</t> Hel, helicase; HE, haemagglutinin esterase; S, spike; E, envelope; M, membrane; N, nucleocapsid; I, internal; CoV, coronavirus; AaCoV, Apodemus agrarius CoV; ChRCoV, China Rat CoV; ApCoV, Apodemus peninsulae CoV; RaCoV, Rattus argentiventer CoV; RtCoV, Rattus tanezumi CoV; MrCoV, Myodes rufocanus CoV; HCoV, Human CoV; MHV, murine hepatitis virus; MERS, middle east respiratory syndrome; SARS, severe acute respiratory syndrome. The sequence of 5′ termini untranslated region was uncovered in AaCoV Jingmen, AaCoV Longquan-343, and RtCoV HMU-1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    Comparison of the genome organization between Apodemus agrarius coronavirus (AaCoV) 1 Aa18–157 and representative betacoronaviruses (β-CoV). The predicted ORFs and conserved domains are represented by boxes in the following colors: orange for non-structural proteins, blue for structural proteins, and green for accessory proteins. AaCoV-1 Aa18–157 and rodent-borne β-CoVs display a genomic structure and gene organization typical of CoVs, arranged from 5′ to 3′ as follows: non-structural proteins located in the ORF1ab region; structural proteins including HE, S, E, M, and N; and accessory proteins such as NS2a, NS4, NS5, and N2. Abbreviations: ORF, open reading frame; NS, non-structural protein; 3CL, 3CL main protease; RdRp, RNA-dependent RNA polymerase; Hel, helicase; HE, haemagglutinin esterase; S, spike; E, envelope; M, membrane; N, nucleocapsid; I, internal; CoV, coronavirus; AaCoV, Apodemus agrarius CoV; ChRCoV, China Rat CoV; ApCoV, Apodemus peninsulae CoV; RaCoV, Rattus argentiventer CoV; RtCoV, Rattus tanezumi CoV; MrCoV, Myodes rufocanus CoV; HCoV, Human CoV; MHV, murine hepatitis virus; MERS, middle east respiratory syndrome; SARS, severe acute respiratory syndrome. The sequence of 5′ termini untranslated region was uncovered in AaCoV Jingmen, AaCoV Longquan-343, and RtCoV HMU-1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: One Health

    Article Title: Epidemiological surveillance and genomic characterisation of betacoronavirus in Apodemus agrarius from Gyeonggi and Gangwon Provinces , Republic of Korea, 2017–2019

    doi: 10.1016/j.onehlt.2026.101381

    Figure Lengend Snippet: Comparison of the genome organization between Apodemus agrarius coronavirus (AaCoV) 1 Aa18–157 and representative betacoronaviruses (β-CoV). The predicted ORFs and conserved domains are represented by boxes in the following colors: orange for non-structural proteins, blue for structural proteins, and green for accessory proteins. AaCoV-1 Aa18–157 and rodent-borne β-CoVs display a genomic structure and gene organization typical of CoVs, arranged from 5′ to 3′ as follows: non-structural proteins located in the ORF1ab region; structural proteins including HE, S, E, M, and N; and accessory proteins such as NS2a, NS4, NS5, and N2. Abbreviations: ORF, open reading frame; NS, non-structural protein; 3CL, 3CL main protease; RdRp, RNA-dependent RNA polymerase; Hel, helicase; HE, haemagglutinin esterase; S, spike; E, envelope; M, membrane; N, nucleocapsid; I, internal; CoV, coronavirus; AaCoV, Apodemus agrarius CoV; ChRCoV, China Rat CoV; ApCoV, Apodemus peninsulae CoV; RaCoV, Rattus argentiventer CoV; RtCoV, Rattus tanezumi CoV; MrCoV, Myodes rufocanus CoV; HCoV, Human CoV; MHV, murine hepatitis virus; MERS, middle east respiratory syndrome; SARS, severe acute respiratory syndrome. The sequence of 5′ termini untranslated region was uncovered in AaCoV Jingmen, AaCoV Longquan-343, and RtCoV HMU-1. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: First and nested PCRs were performed in a 25-μL reaction mixture containing 2.5 U of Ex Taq polymerase (TaKaRa BIO Inc., Shiga, Japan), 2 μg of cDNA, and 10 pmol of each primer.

    Techniques: Comparison, Membrane, Virus, Sequencing